Conjugation

Part:BBa_K1510922:Design

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-09)


Fragrance generator part1: ketoisocaproate decarboxylase (THI3) and RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.



Source

THI3 gene from Saccharomyces cerevisiae and BBa_B0034 from 2014 igem distribution.

References